Journal: Molecular Microbiology
Article Title: Late Stage Mannan Metabolism in Cellvibrio japonicus Requires the Combined Action of a Mannosyl‐Glucose Phosphorylase and a Mannobiose Epimerase
doi: 10.1111/mmi.70043
Figure Lengend Snippet: Growth analyses of C. japonicus single gene deletions, intermediate strains, and double ectopic complementation strains grown in MOPS defined media supplemented with 0.5% (w/v) of either (A) mannobiose, (B) mannotriose, (C) glucomannan, (D) carob galactomannan, (E) guar galactomannan, or (F) linear mannan. Panels A, B, and F were grown on a separate microplate from Panels C, D, and E, and are shown here together for ease of visualization. Growth analyses were conducted using an EPOCH2 microplate reader (Biotek) in biological triplicate for 48 h. Error bars depict standard deviation but are too small to be observed in many of the graphs. Complete growth statistics are available in Table .
Article Snippet: C. japonicus strains were cultured in MOPS minimal medium from TekNova (cat. no. M2106), supplemented with 1.32 mM phosphate and 0.2% (w/v) glucose.
Techniques: Standard Deviation